Evaluation of a Triple Buffered Peptone Broth for Detection of Salmonella in Broiler Feed

The pH of pre-enrichment media containing feed/ingredients can become acidic during incubation due to bacterial utilization feed carbohydrates. This decrease in result cell injury or death, negatively impacting the detection Salmonella. Our objective was evaluate a new triple buffered peptone (TBP) against water (BPW) and lactose broth (LB) for recovery Salmonella from feed. Liquid cultures nalidixic acid resistant strains (Enteritidis, Heidelberg, Kentucky Typhimurium) were added alone, artificially inoculated stored 1 7 d effect medium on Three replicates per treatment conducted. After at 37 °C 24 h, measured prior plating onto brilliant green sulfa agar plates supplemented with 200 ppm (BGSNA). Plates incubated evaluated presence typical colonies. experiment replicated. TBP observed exhibit significantly better buffering capacity than BPW LB. Additionally, able recover 100% time compared (97.9%) LB (61.5%). shows promise maintain neutral which may allow more accurate